Biochrom 30 +

Automatic Amino Acids Analyser

Amino acid analysis

There are several analytical methods that can be used to analyze the amino acids; the Amino Acid Automatic Analyser Biochrom 30, however, unlike the other three including the HPLC, meets the requirements of the AOAC and EU Directive 98/64 / EC of the Commission for the analysis of amino acids from food, fodder, fertilizers and dairy products.

 

ERRECI is pleased to present Biochrom's new range of automatic amino acid analysers:

  • Biochrom 30+, Automated amino acid lithium Analyser (over 50 amino acids in a single chromatographic run), ideal for the free amino acids and physiological samples, (neonatal disorders, nutritional science, pharmaceutical applications, etc.) and dairy products.
  • Biochrom 31+, Automatic Sodium amino acid Analyser (over 24 amino acids), ideal for protein hydrolysates and the food industry (fodder, nutritional science, pharmaceutical applications, etc.) and dairy products.
  • Biochrom 32+, Automatic Sodium amino acid analyser (more than 24 amino acids, in particular separate Methionine and Cysteine), ideal for protein oxidised hydrolysates, the main applications in the food industry (fodder, nutritional science), pharmaceutical applications, etc.

We can point out the main innovations introduced with the new version:

New rapid methods for the analysis of amino acids (lithium) with 90 minute chromatogram for 48 amino acids, a program that saves time (injection-injection) compared to classic working methods.

New, rapid methods for the analysis of amino acids (sodium) with 40 minute chromatogram and 24 amino acids, a program that saves 33% of analysis time

New refrigerated and insulated autosampler.

Improved analytical sensitivity.

Better reproducibility by area and retention time.

The Biochrom 30+ is an instrument dedicated to quantitative and qualitative analysis of all the physiological amino acids, using a cationic exchange chromatography with post-column derivatisation using Ninhydrin. The analytical data is processed using for EZChrom chromatography software.

 

The analysis of amino acids is one of the most important applications in the field of Liquid Chromatography. Its use ranges from routine quality control up to medical research projects, where in any case, a high degree of accuracy and reproducibility in the analysis, is required.

 

Biochrom, with over 25 years of experience in the industry, supplies BIOCHROM 30+, unites and makes the most of the experience accumulated by the company in solving the needs and and problems of its users

 

The Biochrom 30+ is a fully automated analyser, designed specifically for the analysis of Amino Acids and capable of achieving accurate and reproducible results.

 

The system's strengths include:

System and Method built on customer specifications

High level of Applied Technology

Completely inert system

Self-diagnostic system for malfunctions

Full system control via PC

These important considerations are the basis for governing approval from the FDA, which allows the commercialisation of the instrument in the United States

 

The Amino Acid Biochrom 30+ Analyser is designed to ensure accurate quantitative analysis of mixtures of amino acids. The principle behind the method of analysis is a continuous flow chromatography developed by Spacman, Moore and Stein in 1958 and refined and improved by Biochrom to get a fully automatic, fast, responsive analysis system.

This unit uses the latest technology to rapidly obtain accurate and reproducible results with a minimal involvement from the operator. The control software oversees the various analysis operations, monitoring the different control parameters and providing guidance on possible malfunctions in the system (Self-diagnosis system).

The Biochrom 30+ package consists of 4 operating units, one regarding electronics, one regarding the pumping system, one regarding the detection system and the last one relative to the automatic self-sampler with automatic Peltier temperature control.

The system is also supplied with:

Analytical column in resin for the separation of Amino Acids

Precolumn

Operating system complete with interface

Reagents and calibration standards

EZChrom control / acquisition software for Biochrom 30

Ninhydrin Reagent and Swab Solutions

Instruction manual

Package of instruments for essential operations

Package of accessories and Consumables

Power Cables

 

The analysis of amino acids is one of the core activities in the field of Liquid Chromatography.

Its use ranges from routine quality control up to medical research projects, where in any case, a high degree of accuracy and reproducibility in the analysis, is required.

Biochrom, with over 25 years of experience in the industry, supplies BIOCHROM 30+, which unites and makes the most of the experience accumulated by the company in solving the needs and and problems of its users

The Biochrom 30+ is a fully automated analyser, designed specifically for the analysis of Amino Acids and capable of achieving accurate and reproducible results.

The system's strengths include:

•  System and method built on customer specifications

• High level of Applied Technology

• Completely inert system

• Self diagnostic system for malfunctions

• Complete system control via PC
These important considerations are the basis for governing approval from the FDA, which allows the commercialisation of the instrument in the United States

Operating Principle

Ion chromatography with post-column derivatisation

The sample, containing a mixture of amino acids, is injected in a cation exchange resin column, the eluents (Swab solutions with different pH and ionic strength) are pumped through the column separating the different amino acids based on the specific affinity with the mobile phase and the stationary phase.

The column temperature is carefully controlled and can be changed according to the specific separation needs to be obtained.

The eluent-sample mixture reacts with Ninhydrin, in a specific high-temperature reactor.

In the reactor, the Ninhydrin reacts with amino acids found in the eluate, forming a coloured compound.

The intensity of the coloured compound (Amino Acid + Ninhydrin) product is directly proportional to the amount of this amino acid.

From the reactor, the eluate / Ninhydrin amino acid mixture is pushed towards the photometric detector, where each coloured compound (Amino Acid + Ninhydrin) is quantified by measuring the amount of light absorbed.

The Absorbance is measured at two wavelengths (570 and 440 nm) because some amino acids, linked with Ninhydrin, absorb at 440 nm while others absorb at 570 nm.

The output signal from the photometer is sent to an integration system that collects the chromatogram and calculates the concentration of amino acids, shown as a series of peaks.

The retention time of the peak, identifies the amino acid from the qualitative point of view, while its area is used to obtain the concentration.

To obtain a precise analysis, the system must be calibrated with a mixture of standard amino acids, from which the data relating to our sample can be derived at a later date.

After each analysis, the column is regenerated with a strong base followed by the "Swab 1" solution that re-balances the column before the next analysis.

All operations are performed AUTOMATICALLY by the Biochrom 30+ and the operator only has the task of viewing functions and parameters via the control software.

The system is supplied with different analytical methods (standard programs) that contain all the necessary information for carrying out the analyses, such as:

The choice of the swab solutions is via the control of the valves for the formation of the gradient

The pump control parameters for the swab solutions and the pump for the Ninhydrin

The parameters for the injection of samples

The temperature of the reactor and of the column

The analysis time

The acquisition parameters and integration of the chromatogram

The electronic part of the instrument is protected with different security systems. The Biochrom 30+ is made up of two separate units:

Management unit for the eluents

Autosampler unit

In relation to the methods of sample preparation and related analytical results, we should mention:

Free amino acids: it is possible to determine the free amino acids without any hydrolysis, you only need to deproteinize the sample once.

Total amino acids via acid hydrolysis: determination of 15 amino acids

Tryptophan cannot be established for loss during hydrolysis

The Methionine / Cystine couple is determined as the sum of the two amino acids (due to overlapping)

Glutamine determined as the sum of glutamine / glutamic acid

Asparagine determined as the sum of asparagine / aspartic acid

basic hydrolysis for the determination of Tryptophan: total determination of the tryptophan via basic hydrolysis.

Oxidation plus acid hydrolysis: Separate Methionine and Cysteine

Automatic amino acid analyser

Biochrom 30/30+

Biochrom 30+

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